KMID : 0620920140460100003
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Experimental & Molecular Medicine 2014 Volume.46 No. 10 p.3 ~ p.3
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Chronic stress enhances progression of periodontitis via ¥á1-adrenergic signaling: a potential target for periodontal disease therapy
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Huaixiu Lu
Minguang Xu Feng Wang Shisen Liu Jing Gu Songshan Lin
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Abstract
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This study assessed the roles of chronic stress (CS) in the stimulation of the sympathetic nervous system and explored the underlying mechanisms of periodontitis. Using an animal model of periodontitis and CS, the expression of tyrosine hydroxylase (TH) and the protein levels of the ¥á1-adrenergic receptor (¥á1-AR) and ¥â2-adrenergic receptor (¥â2-AR) were assessed. Furthermore, human periodontal ligament fibroblasts (HPDLFs) were stimulated with lipopolysaccharide (LPS) to mimic the process of inflammation. The proliferation of the HPDLFs and the expression of ¥á1-AR and ¥â2-AR were assessed. The inflammatory-related cytokines interleukin (IL)-1¥â, IL-6 and IL-8 were detected after pretreatment with the ¥á1/¥â2-AR blockers phentolamine/propranolol, both in vitro and in vivo. Results show that periodontitis under CS conditions enhanced the expression of TH, ¥á1-AR and ¥â2-AR. Phentolamine significantly reduced the inflammatory cytokine levels. Furthermore, we observed a marked decrease in HPDLF proliferation and the increased expression of ¥á1-ARfollowing LPS pretreatment. Pretreatment with phentolamine dramatically ameliorated LPS-inhibited cell proliferation. In addition, the blocking of ¥á1-ARsignaling also hindered the upregulation of the inflammatory-related cytokines IL-1¥â, IL-6 and IL-8. These results suggest that CS can significantly enhance the pathological progression of periodontitis by an ¥á1-adrenergic signaling-mediated inflammatory response. We have identified a potential therapeutic target for the treatment of periodontal disease, particularly in those patients suffering from concurrent CS.
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KEYWORD
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